My Life Simulator

My Life Simulator Chapter 361

2021-10-14

Chapter 360 Lu Li, a man leading the times

Since the virtual gene chip, the progress of the experiment has accelerated significantly.

Detecting gene expression information is like doing a puzzle. At first is very difficult, and as more and more gene fragments are identified, the speed will become faster and faster.

Seeing the progress of the experiment in the virtual lab, Lu Li nodded slightly. According to the current process, the human genetic map should be completed within a few years.

Gene testing is going on, but gene-targeting technology is not going anywhere.

Before the completion of the human genetic map, it is really difficult to improve the gene positioning from the software, that is, the accuracy of the information.

Perhaps... there is something wrong with my thinking.

Since the ability of transcriptase is not good, then change to another ability!

Thinking to this, Lu Li suddenly became enlightened.

Transcription activators, like effectors (TALEs), with gene targeting specificity in human cells, are not irreplaceable!

There must be other undiscovered "enzymes" that can replace the function of transcriptase.

So, Lu Li began to study various cells, bacteria and viruses.

Animal cells, plant cells, bacteria, and even viruses, analyze cell structures and find enzymes with gene targeting specificity.

This process has not been smooth.

It is a complete try one's luck to search for enzymes with target specificity in various protein molecules.

As the days go by, the enzymes with target specificity remain undiscovered.

Liu Qin still came to Fudan. Just like in reality, Liu Qin became an Associate Professor of Fudan Economics Institute.

It's just...the difference from reality is that before Liu Qin joined Fudan, he underwent a strict and meticulous political review.

Because when Liu Qin was a Ph.D. student at Harvard, interpersonal relationships were relatively simple, and there were no suspicious social relationships. Moreover, Father Liu's business is also based in China, with no foreign assets at all, and no business dealings with foreign countries.

So... there is no problem with Liu Qin's political trial.

When Liu Qin told Lu Li the news of the political trial, Lu Li was helpless. It turns out that when I fall in love with someone now, I still need to go through state censorship.

The arrival of Liu Qin has enriched Lu Li's weekend time, and he is no longer in the laboratory every weekend.

Professor He is very pleased with this. Scientists are also human, and they cannot regard themselves as machines. Maybe the arrival of love can inspire more inspiration.

These words... turned out to be true!

In the second month after Liu Qin joined Fudan, Lu Li's research finally achieved a key breakthrough.

He found another enzyme with target specificity - nickase!

In type II restriction enzymes, there is also a special type, the enzyme only cuts one strand of double-stranded DNA, resulting in a nick, such restriction enzymes are nickases.

From the functional analysis, transcriptase is a large-scale search radar, and nickase is a fire control radar.

In terms of positioning accuracy, the nickase is indeed very accurate, and it is almost always accurate. The problem is... without a large-scale search radar, it is difficult for the fire control radar to find the target.

So...then combine the two.

So, Lu Li started experimenting.

Prepare spliced protein, prepare transcriptase, and prepare nickase, and then combine the three to form a brand new gene editing tool.

Next, Lu Li entered the guide RNA on the transcriptase to determine the characteristics of the target gene. Then use the gene gun to inject the new gene editing tool into the cells to be edited.

Under the electronic Microscope, the guide RNA on the transcriptase begins to search for the target on the gene chain, and the guide RNA matches the target gene to initially lock the approximate orientation of the target.

Immediately, the cleavage protein begins to move towards the target orientation.

When the shear protein moves to the position where the target is, the nickase fire control radar is turned on, the accurate target is instantly calibrated, and the target is locked!

In the next instant, the spliced protein moves to the target position and begins to fuse with the gene chain, and the helical gene chain is unwound and flattened.

Then... as if "ka-cha", the cleavage protein cut exactly the marked position.

Good start! The first experiment was successful!

Afterwards, Lu Li repeated the same experiment hundreds of times, and the results of each experiment were all 100% without the slightest error!

It worked!

The combination of scanning radar and fire control radar allowed Lu Li to create a whole new gene editing tool.

Gene targeting technology achieves a revolutionary breakthrough!

Since then, human gene editing technology has reached a new level.

The new gene editing tool can accurately lock the target and cut the required gene fragment accurately, no need to try one's luck anymore.

Lu Li closed the virtual lab with a big smile on his face.

Gene technology has achieved another major breakthrough!

Back in the study, Lu Li sat down at the computer desk and started writing the paper.

“Gene targeting technology, a brand-new gene editing tool.”

In the paper, Lu Li described the a new gene editing tool.

In the original cleavage protein + transcriptase gene editing technology, the nickase "fire control radar" has been creatively added, which makes this brand new gene editing technology with extremely high gene targeting. The accuracy rate and gene editing efficiency have been improved by thousands of times.

After writing the paper and printing it out, Lu Li walked out of the office with the paper materials.

"Teacher, I wrote a new paper, please take a look."

When he came to Professor He's office, Lu Li handed over the thesis materials in his hand.

"New paper? You...have produced results again?"

Professor He sighed inwardly, "It's so scary", and reached out to take the paper handed over by Lu Li .

"Gene targeting technology? A new gene editing tool?"

Seeing the title of the paper, Professor He sucked in a cold breath of air, uh... blown out of the air conditioner air-conditioning!

Lu Li came up with a new gene editing tool?

Suppressing the shock in his heart, Professor He buried his head and looked towards the thesis materials in his hand.

After reading it carefully, Professor He has clearly seen from the presentation of the paper that a gene editing technology with higher efficiency and higher accuracy has been officially born.

With this new gene editing tool, human gene editing technology has reached a new level.

Lu Li...he really is a genius!

First, a brand new anti-cancer technology was developed, and now a brand new gene editing tool has been developed.

The pillar of the state, the blessing of the society!

Lu Li...will lead the future!

Looking up and looking towards Lu Li in front of him, Professor He suppressed his excitement and said to Lu Li: "Go! Let's do a verification."

"Okay! "

Said, the two walked out of the office together and came to the laboratory.

In the laboratory, Lu Li repeated the experimental process in the virtual laboratory again, and performed one hundred gene editing experiments in a row.

The success rate is 100%, and the accuracy rate is 100%.

"It's amazing! It's amazing!"

Professor He picked up the laboratory verification data, his hands were shaking, "The efficiency of gene editing has been improved thousands of times. . This is a revolutionary breakthrough!"

Lu Li smiled.

"This paper..."

Professor He raised his eyes and looked towards Lu Li, and said seriously: "Delete specific experimental steps, delete detailed experimental data, and only make theoretical statements. Revise it and send it to the world's top academic journal 'Nature'."

Speaking, Professor He looked at the paper in his hand again, and said, "I submit this paper to you. Chinese Academy of Sciences, as an internal document."

"Okay!"

Lu Li agrees with Professor He's practice of "keep one hand".

Science has no borders, but scientists have nationalities!

We master the latest technological achievements by ourselves, as for others... give you a direction, go and study it yourself!

Subsequently, Lu Li revised the paper and wrote a directional and theoretical paper. It's just... At the end of the paper, Lu Li attached the laboratory's verification conclusion.

After submitting the manuscript to the "Nature" journal, Lu Li posted his paper on the academic website again.

…

Across the Pacific Ocean.

In the Millikin Institute of Genetics, researchers are busy and busy.

In the research of genetic technology, the Millikin Institute of Genetics has enough confidence that they have been at the forefront of the world.

The concept of gene chip technology was put forward by them.

The gene editing technology CRISPR-Cas9 was also developed by them.

Although Lu Li's new generation of anti-cancer technology has achieved success, it is only a result of technical application, and the foundation is CRISPR-Cas9 gene editing technology.

It's just... CRISPR-Cas9 technology, the efficiency is still too low!

Inaccurate gene mapping and off-target gene editing requires thousands of experiments to obtain a correct specimen.

So...the chief scientist at the Millikin Institute for Genetics, Steven, is not happy about it.

"We are looking for a new gene editing tool. I will solve the funding problem. Even if it costs tens of billions of dollars, we must develop a new gene editing tool within three years. Tools. We...lead the world!"

In this morning's meeting, Steven Academician's voice was impassioned.

And then...his speech was interrupted by a scream!

"Omg!"

A man with sparse hair and deep hairline looked at the screen of his mobile phone and exclaimed: "Steven Academician, you You'd better look at the academic website first! Someone has already developed this brand-new gene editing tool!"

"What?" On the mobile phone, I opened the academic website, and I saw a paper that caused a heated discussion.

"Gene targeting technology, a new gene editing tool."

The author...Lu Li!

Is it him?

Steven's heart skipped a beat, and he hurriedly opened the paper and read it.

The paper theoretically describes a new combinatorial module of gene editing tools, cleavage protein, transcriptase, plus a new "targeting enzyme".

Targeted by "targeting enzymes" to achieve accurate gene editing.

This kind of theoretical paper is actually not of much value, and it is almost enough to read it.

However...at the end of the paper, Lu Li even attached a laboratory verification report, confirming that the accuracy of the new gene editing tool is as high as 100%!

This proves that the new gene editing tool developed by Lu Li has been successful!

But...what kind of enzyme is this "targeting enzyme"?

Falco! Why not write down what enzyme is it?

Steven Academician jumped with anger, slapped his phone on the table with a "Pa" sound, and pointed at the man with a deep hairline who exclaimed before, "You, this month's bonus is not enough. I'm done!"

"Why?"

The man with the deep hairline was sluggish.

"Because...you're playing on your phone in a meeting!"

Well, that reason...that's powerful!

A man with a deep hairline curls one's lip slightly, Steven Academician, you should be flying into a rage out of humiliation, right?

 …

This unpublished paper by Lu Li has already aroused intense repercussions in the academic circles.

Countless people looked at that "targeting enzyme" gnashing teeth. What exactly is a targeting enzyme? Why did you write it for me!

There are tens of thousands of known enzymes. There are countless variants of each enzyme. If you don't write it out, how do we find this targeting enzyme?

Science knows no borders, why is it so narrow? Announce it! Publish it to me!

……

On the evening of the second day, another news about Lu Li was broadcast on Huaxia TV's news network.

"News from this station: Lu Li Academician, a famous scientist in my country, after conquering cancer, has researched a world-leading scientific research achievement and created a brand-new gene editing tool with a high accuracy rate of gene editing. Up to 100%, the gene editing efficiency has increased by 10,000 times."

"The success of this technology research indicates that my country has reached the world's leading level in the field of gene editing."

After the news was broadcast, people watching TV had only one feeling in their hearts, Lu Li again! It's Lu Li again!

The online media also followed up immediately, reporting on Lu Li's world-leading new technology.

"Lu Li, the pride of the Chinese people!"

"Leading the world again, cheers for Lu Li!"

"Lu Li has created a A brand-new gene editing technology."

"The great breakthrough of gene technology, Lu Li has made gene editing technology leap forward."

"This man once again leads the world and leads the times !"

In the continuous reports on TV, newspapers, and the Internet, Lu Li has just dropped his popularity and once again became popular all over the country, name shakes the whole world.

"Your surname is Lu, you sure are 666!"

"God, please accept my knee!"

"I'm writing 'a new generation of gene editing' The graduation thesis of "Technology Imagination"! You have already researched it. How can I still graduate!"

"Sympathy for the wave upstairs. Besides...I can't help laughing out loud. ?"

"As a scientific researcher in genetics, let me talk about the importance of this technology here. The emergence of this technology is similar to the invention of the steam engine. Genetic engineering has entered the A new era!"

"The next era, the genetic era. Lu Li leads the genetic era for us!"

"Lu Li, the man who leads the era!"

(end of chapter)