My Life Simulator

My Life Simulator Chapter 354

2021-10-14

Chapter 353 Is this a major breakthrough?

Removing the specimen from the human body, Lu Li walked out of the operating room.

When he came to a test bench next to him, Lu Li sat down and started to do gene editing of T cells.

Gene editing and modification, the technology has been around for a long time.

Gene modification by natural means, including Fruit Tree grafting technology, and even the well-known hybrid rice technology, these are all gene modification technologies.

People are also familiar with gene editing and modification by unnatural means, such as...transgenic technology.

Even if it is to manipulate human genes, it is not one or two times.

As early as 2016, a guy named He Jiankui manipulated human embryos to create a pair of so-called "AIDS-immune" gene-edited babies, and then... He Jiankui was arrested. Jailed!

Under the condition that the technology is completely immature, a pair of gene-edited babies is actually produced. This is so...is there any conscience and morality of a scientist?

If genetic technology is a road to the sky, human beings have only just taken a small step, so they want to play God? How stupid, how arrogant is this?

The more advanced human science and technology are, the more we must maintain awe.

Lu Li sat in front of the test bench, cautiously took out a little cancer cell sample and installed it under the electronic Microscope.

In the image of the electronic Microscope, the internal structure of the cell is clearly displayed on the display screen.

Endoplasmic reticulum, ribosome, Golgi apparatus, lysosome, mitochondria, microsomes, vacuoles, cytoskeleton, cytosol, and even DNA entangled inside the nucleus are clearly presented.

DNA requires an electronic Microscope to see, but... if you straighten it, the DNA strands are over two meters long.

Manipulating the instrument and using a gene gun to inject Cas9 transcriptase into cancer cells, Lu Li clearly saw that the transcriptase was close to the DNA chain and copied a piece of DNA information.

The transcription of cancer cell gene information is completed.

Lu Li started another experiment. The T cells were installed under the electronic Microscope, and Lu Li used a gene gun to inject the gene information of cancer cells into the T cells.

Soon, T cells absorb this genetic information and incorporate it into the "hostile sequence" of the immune system.

Do this step, and the weapon against cancer cells has been prepared.

But... once injected into the human body, the T cells will divide wildly, which will inevitably bring the immune system crazy, and kill the enemy regardless of the enemy.

As a result, the immune system kills the body before cancer kills it.

So, Lu Li has to do one more step.

At this time, Lu Li plans to modify the "information dissemination mechanism" of T cells, so that it will not be "air support" for summon, nor "call friendly forces" everywhere, and only let it fight alone.

Perhaps, this way it might not trigger the immune system to go mad.

This in turn involves another gene editing technology, CRISPR-Cas9.

This gene editing technology is derived from the mutation mechanism of bacteria against phages.

When bacteria fight phage attack, they mutate a special protein that cuts off the gene chain that the phage injects into the nucleus.

It has to be mentioned here that bacteriophages are the most terrifying killers in this world.

Every moment, in every corner of the world, a war that has lasted for countless years is breaking out. The scale of the war has reached a terrifying scale of trillions of magnitudes.

This is the phage attack on bacteria.

Bacteriophage is a virus, its shape is very regular, its head is a twenty-sided dice, it has a small stick-like torso, and there are a few spider legs below it, like a nano-robot .

Bacteriophages are harmless to the human body, but just frantically attack bacteria. They inject their own DNA into the bacteria and divide into countless phages inside the cell, killing the bacteria and spreading.

In the face of phage attack, bacteria have also evolved a means of resistance, which is a special protein mentioned above. This protein cuts off the DNA injected by the phage.

Humans use this special protein to cut genes, modify genes, and even edit genes.

In the laboratory, Lu Li repeated the experiment of phage attacking bacteria, and obtained the special protein CRISPR that bacteria use to fight phage.

The CRISPR protein, combined with the Cas9 transcriptase, was then injected into the T cells to modify the genes of the T cells.

Gene mapping is a problem.

Lu Li has done countless experiments, but failed to obtain accurate genetic modification effect.

Even though transcriptases will match genes, however... splicing proteins often cut in the wrong place.

Manipulating genes is not about cutting them with scissors. This extremely microscopic operation cannot penetrate into the nucleus with any tool. This operation can only be achieved by cutting proteins.

Fortunately, the gene match of the transcriptase is still in play.

After repeating the experiment hundreds of times, I finally got it right once.

A gene-edited T cell was finally born.

Lu Li got up quickly and injected this T cell into the virtual human body.

And then there's the wait!

After the gene-edited T cells enter the human body, they immediately detect the existence of "hostile target" cancer cells, instantly activate the stress mechanism, begin to absorb nutrients from the human body, and divide frantically.

After half an hour, the experimental body began to have a fever, and the heart rate increased sharply.

This is the immune mechanism at work. The symptoms of fever are the normal mechanism of the immune system to deal with virus invasion.

And then...the immune system went crazy!

Even if T cells do not "call friendly forces", the deployment of the army of T cells will still lead to a violent immune response in the human body, and the "factor storm" still occurs.

It's just...it doesn't seem to be that serious.

Lu Li hastily created a medicine that controls the madness of the immune system to maintain the lifeform of the experimental human body.

Heart injections, adrenal hormones, first aid, and various emergency measures have been used one after another.

"Sure enough, my thinking is right!"

Lu Li put out a long breath and grinned, "Eliminating the ability of T cells to call friendly forces can reduce the immune system's madness ”

Although this little progress has not yet made a key breakthrough in CAR-T cancer treatment technology, but...as a graduate thesis, the real value is absolutely enough.

After closing the virtual lab, Lu Li opened his eyes, and another night passed.

After getting up to wash up, go out to eat breakfast, Lu Li returned home, sat in front of the computer, and started writing the thesis.

“CAR-T technology, a preliminary study on the information transmission mechanism of shielded T cells!”

Lu Li sat in front of the computer desk, cracking with both hands on the keyboard, a detailed data, The experimental steps are clear, authentic and reliable papers, easy to write.

One Chinese, one English, all done.

Then, Lu Li called Professor He, "teacher, my research topic has been..."

"I have already thought about the topic? It's very efficient!"

Before Lu Li could finish speaking, Professor He took over, "Okay, you can send me the subject! I'll report it to you."

"That It's not a project, it's a paper. I wrote a paper."

Lu Li quickly explained: "teacher, I wrote a paper and sent it to your mailbox. Please correct me! "

"Thesis?"

Professor He was stunned for a moment. You didn't even choose a topic, so you wrote your thesis? Is it the kind of irrigation paper with a mixed name?

"I'll take a look!"

Professor He didn't say much, and was ready to read it first. If it is really a watering paper, then you should take care of Lu Li and kill this malignant social trend.

Sit back at the desk, Professor He turned on the computer and saw the paper sent by Lu Li in the mailbox.

When I opened the paper and saw the title of "CAR-T technology, a preliminary study on the mechanism of shielding T cell information transmission", Professor He was taken aback for a moment.

CAR-T technology? This is still a cutting-edge scientific research project in the world!

A mechanism for screen T cell information transmission? New gene editing technology?

Professor He suddenly looked solemn and looked at it seriously.

The paper is in duplicate, one in Chinese and one in English. Professor He didn't bother to read the English version, so he picked up the Chinese version of the paper and read it.

There is no moisture in the whole paper, it is all dry goods.

In the paper, Lu Li described the new direction of gene editing in T cells in detail. Every step is clear and the experimental data is also very detailed.

From the perspective of the paper, as long as Lu Li's experimental steps are followed, the gene editing technology that shields the information transmission mechanism of T cells is completely repeatable.

Theoretically, shielding the information transmission mechanism of T cells can indeed effectively reduce the "factor storm" and reduce the probability of the immune system going crazy.

"This kid... silently, he actually set a bomb on me!"

Professor He had a bright smile on his face, "CAR-T technically This is a major scientific research achievement! Not bad! Not bad!"

After reading the paper, Professor He hurriedly downloaded the paper and printed it out. Then, Professor He picked up the thesis materials, turned around and ran out.

All the way to Fudan Medical College Tumor Research Office, I found Professor Guo who is engaged in tumor medical technology research.

"Lao Guo, Lao Guo, take a look at this paper."

Professor He shouted, showing the materials in his hand to Professor Guo.

"Thesis?"

Professor Guo from the Oncology Laboratory looked up at Professor He, "Old He, you are so excited, does this paper still have some goods? "

"More than just in stock?"

When he came to Professor Guo, Professor He handed over the paper, "See for yourself!"

" CAR-T technology, a preliminary study on the mechanism of shielding T cell information transmission? The author...Lu Li?"

Seeing this title, Professor Guo immediately became serious when he saw the author, "It turned out that Lu Li wrote Yes. This kid, actually researched on this? It's amazing!"

Picking up the paper, Professor Guo sat down and read it seriously.

After reading it all over again, a look of surprise appeared on Professor Guo's face, "From the point of view of the experimental steps and ideas, this new direction of gene editing has actually... succeeded?"

"It looks like this!"

Professor He grinned, "I came to you just to repeat this experiment."

"Okay!"

Professor Guo got up quickly, "I also want to verify it. If the experiment can be repeated, then...that would be amazing!"

A master student actually came up with this kind of real value pole. It's amazing that Gao's papers have achieved such a breakthrough in cutting-edge scientific research projects.

As a result, Professor Guo ordered all the master students and Academician students in the tumor laboratory to hand out a paper to them, and asked them to repeat Lu Li's experiment.

"Lu Li's paper? CAR-T technology?"

After seeing this paper, Academician students and master students in the laboratory were dumbfounded one by one.

This guy Lu Li has just been in graduate school for less than a year, right? This has come up with such scientific research results?

Originally, Lu Li's previous "asks" made Professor Guo praise Lu Li all the time, which made everyone feel embarrassed.

Now... how come you actually got results?

Doesn't make people live?

I slandered in my heart that these "research dogs" could only repeat Lu Li's experiments according to Professor Guo's instructions.

Everyone acted immediately, like an assembly line, constantly preparing cleavage proteins, preparing transcriptases, and constantly injecting them into T cells.

Compared with Lu Li, who does all the steps by himself, this pipeline mode is much more efficient.

In an hour, more than 300 T-cell genetically modified samples have been prepared in the laboratory.

Among the more than 300 specimens, four T cells that can accurately shield the information transmission mechanism appeared.

The experiment was successful!

"Hahahaha! It really worked!"

Professor He couldn't help laughing out loud, "Lu Li really did it! Hahahaha!"

The surrounding graduate students and Academician students were silent.

Professor Guo looked at the successfully prepared T cells, and then looked at Professor He, who was laughing from ear to ear, and sighed inwardly.

How could such an outstanding student fall into the hands of Lao He? Really walked the lucky dog excrement!

Afterwards, Professor He sent out Lu Li's paper and submitted it to the core journal "Chinese Medicine", and attached the repeated test records of Fudan University.

The editorial department of "Chinese Medicine" magazine, after receiving this submission, was surprised to see the title, and then saw the repeated experiment records of Fudan University attached, and said nothing. Go directly to the reviewers.

Fudan University has already verified it once, which is clearly a scientific research result that has been tested by practice.

There are several reviewers, some are professors of medicine at Yenching University, and some are academicians of the Chinese Academy of Sciences.

After receiving the manuscript, the reviewers read it seriously.

After reading Lu Li's paper and seeing the repeated test records of Fudan University, the reviewers were amazed again.

Is the author a graduate student?

A master student actually came up with such scientific research results?

How powerful is Fudan's scientific research strength?

With a sigh, the reviewer arranged for a repeat experiment. The results... once again proved that Lu Li's paper was absolutely correct.

Not long after, the journal "Chinese Medicine" officially published this paper by Lu Li.

Once the paper came out, it caused a huge shock in the scientific community and also caused a huge repercussions in the society.

"To conquer cancer is no longer a dream. Chinese scientist Lu Li has achieved a major breakthrough in CAR-T anti-cancer technology!"

Lu Li: Is this a major breakthrough?

(end of this chapter)